Ginsenoside CK Inhibits TGF-β-Induced Epithelial-Mesenchymal Transition in A549 Cell via SIRT1

Figure 5

Involvement of SIRT1 in TGF-β-induced EMT inhibited by ginsenoside CK. A549 cells were treated with TGF-β, with or without 1.5 μg·mL^-1 or 3 μg·mL^-1 ginsenoside CK for 24 h, and the protein expression of SIRT1 was determined by Western blotting (a) and immunofluorescence staining (b). (c) A549 cells were transfected with SIRT1 plasmid with or without 1.5 μg·mL^-1 or 3 μg·mL^-1 ginsenoside CK for 24 h. Representative Western blotting images for vimentin and E-cadherin in A549 cells are shown. (d) Cells were treated with the SIRT1 inhibitor EX-527 and then further incubated in the presence of ginsenoside CK for 24 h. The expression of E-cadherin, vimentin, and SIRT1 was determined by Western blotting. (e) The expressions of SIRT1, E-cadherin, and vimentin were quantified by Gray analysis. Error bar, SD of three independent experiments. .

(a)

(b)

(c)

(d)

(e)