Curcumin Administered in Combination with Glu-GNPs Induces Radiosensitivity in Transplanted Tumor MDA-MB-231-luc Cells in Nude Mice

Figure 1

Cell culture and model establishment. (a) MDA-MB-231-luc cells were imaged (magnification, ×100; scale bar, 5 μm). (b) The bioluminescence intensity of different numbers of cells (, , and ) was detected using an in vivo imaging system in a black opaque 96-well plate. (c) A breast tumor-bearing mouse model exhibiting stable luciferase expression was successfully established 3-7 days after inoculation. (d) Schematic diagram of xenografts in nude mice. MDA-MB-231-luc cells were transplanted into the second pair of subcutaneous mammary glands on the left-hand side. When the tumor volume reached 100-200 mm³, curcumin in corn oil (2% DMSO), Glu-GNPs, and cisplatin in saline solution were administrated regularly. After 3 weeks of treatment, tumor-bearing mice (IR, Glu-GNPs+IR, Cur+IR, and Cur+Glu-GNPs+IR) were treated with irradiation (10 Gray). After further 3 days, the fluorescence intensity of mice in each group was detected using the in vivo imaging system. Glu-GNPs: glucose-gold nanoparticles; IR: irradiation; Cur: curcumin.

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