Curcumin Administered in Combination with Glu-GNPs Induces Radiosensitivity in Transplanted Tumor MDA-MB-231-luc Cells in Nude Mice

Figure 4

Detection of tumor tissue samples. (a) Representative images of HE staining (magnification, ×400) depicting the degree of malignancy in xenografts and tumor cells. The cells of tumor tissues exhibited large and dark nuclei with almost no cytoplasm and centrally located necrosis. Arrows indicate tumor cells (scale bars, 20 μm). RT-qPCR analyses of (b) HSP90 and (c) VEGF mRNA levels in tumor tissue. β-Actin was used as a reference gene. Data were presented as the of three independent experiments. vs. the model group (Student’s -test). (d) Representative images of HSP90 and VEGF IHC staining (magnification, ×400). Tumor tissue was immunostained using DAB (brown) and hematoxylin (blue) for nuclear counterstaining. Arrows indicate the nuclear expression of HSP90 or VEGF in breast cancer cells (scale bars, 20 μm). (e) The semiquantitative scoring analysis of HSP90 and (f) VEGF protein expression in tumor tissue. HE: hematoxylin and eosin; HSP90: heat shock protein 90.

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