SOCS1 Mediates Berberine-Induced Amelioration of Microglial Activated States in N9 Microglia Exposed to β Amyloid

Figure 3

Berberine decreased IL-1β and IL-6 releases in Aβ-treated microglia, and SOCS1-siRNA or SC-siRNA did not induce obvious inflammation in untreated microglia. N9 microglial cells were divided into four groups, including the normal cultured control group, 5 μM Aβ exposure group, BBR treatment group (medium containing 5 μM BBR and 5 μM Aβ), and 5 μM BBR treatment group. After 24 h treatments, ELISA was taken to assess IL-1β and IL-6 concentrations in the medium. Then, the cells were divided into three groups, including control, SOCS1-siRNA, and SC-siRNA groups; after 6 h incubation, Western blot and ELISA were taken to evaluate the SOCS1 expression and TNF-α release. (a) IL-1β concentration in the medium (). (b) IL-6 concentration in the medium (). (c) SOCS1 expression level (). (d) The siRNA-induced effects on TNF-α release (). Results are expressed as . ; NS: no significance.

(a)

(b)

(c)

(d)