Research Article

Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering

Figure 2

Live-dead staining study. Live cells stained green with calcein-AM, dead cells stained red with propidium iodide (PI), and nuclei stained blue with Hoechst. (a) SMC spheroids remain live until day 7 of culture. (b) SMCs in 2D as control show no dead cells until day 7 of culture. Scale bar is 100 μm.
(a)
(b)