Research Article
Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering
Figure 4
Immunofluorescent staining. SMC spheroids and 2D (monolayer) SMCs stained positive for SMC lineage-specific markers α-smooth muscle actin, calponin, smoothelin, and myosin heavy chain 11. Bladder wall ECM proteins collagen I, collagen III, elastin, and fibronectin are more strongly expressed in SMC spheroids. Nuclei stained blue with DAPI. Scale bar is 100 μm.