PICK1 Deficiency Exacerbates Sepsis-Associated Acute Kidney Injury

Figure 3

Downregulation of PICK1 aggravated LPS-induced apoptosis of HK2 cells. (a, b) The levels of PICK1 mRNA and protein were detected by qPCR and WB (NC versus sh-PICK1, ). (c) The expression levels of apoptosis-related proteins Bcl-2, Bax, and cleaved caspase 3 were detected by western blotting (NC versus sh-PICK1, ). (d) Representative cleaved caspase 3 level detected by immunohistochemistry (the control versus LPS, LPS versus LPS+FSC231; , ). (e, g) Representative immunofluorescence results (WT+LPS versus NC+LPS, NC+LPS versus sh-PICK1+LPS; ). (f–h) The apoptosis of HK2 cells was detected by Hoechst 33258 staining (magnification ×200,  μm, WT+LPS versus NC+LPS, NC+LPS versus sh-PICK1+LPS; ). (f, h) Representative Hoechst 33258 staining results (WT+LPS versus NC+LPS, NC+LPS versus sh-PICK1+LPS; ). (i) The apoptosis of HK2 cells was detected by CCK-8.

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