miR-101-3p Serves as a Tumor Suppressor for Renal Cell Carcinoma and Inhibits Its Invasion and Metastasis by Targeting EZH2

Figure 3

EZH2 was the downstream target of miR-101-3p. (a) The diagram of miR-101-3p potential target genes predicted by TargetScan, miRTarBase, and miRDB. (b, c) Schematic of EZH2 wild-type (WT) and mutant (Mut) luciferase reporter vectors. (d) Relative luciferase activity measured by luciferase assays in HEK293T cells cotransfected with miR-101-3p mimics or NC. (e) Pearson’s correlation coefficient analysis of miR-101-3p expression levels with EZH2 in starBase. (f, g) Relative expression of EZH2 in unpaired or paired RCC tissues based on starBase and GEPIA databases. (h–j) Kaplan-Meier survival analyses of EZH2 expression with poor survival. Data indicate of three experiments ().

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