Development of Rapid and Visual Nucleic Acid Detection Methods towards Four Serotypes of Human Adenovirus Species B Based on RPA-LF Test

Figure 4

Optimization of reverse primer and probe concentrations in reaction systems of (a) RPA-HAdV, (b) RPA-HAdV3, (c) RPA-HAdV7, (d) RPA-HAdV21, and (e) RPA-HAdV55. The concentrations of reverse primers and probes used here are indicated. The top and bottom red lines on each strip are the control and test lines, respectively. (−): bad result; (+): good result; (++): excellent result.

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