Development of Rapid and Visual Nucleic Acid Detection Methods towards Four Serotypes of Human Adenovirus Species B Based on RPA-LF Test

Figure 5

Optimization of amplification time of RPA-HAdV in detecting plasmid HAdV3-Hexon-pMD18T (1) and HAdV55-Hexon-pMD18T (2) and of RPA-HAdV3 (3), RPA-HAdV7 (4), RPA-HAdV21 (5), and RPA-HAdV55 (6) in detecting their corresponding positive plasmids. The top and bottom red lines on each strip are the control and test lines, respectively. Various amplification times are indicated. (−): negative result; (+): positive result.