Development of Rapid and Visual Nucleic Acid Detection Methods towards Four Serotypes of Human Adenovirus Species B Based on RPA-LF Test

Figure 6

Evaluation of the detection limit of each RPA-LF assay in detecting (a) positive plasmids or genomic DNA of (b) HAdV 3, (c) HAdV 7, (d) HAdV 21, and (e) HAdV 55. 1, 2, 7, 9, 11, and 13 represent detection results of various concentrations of plasmids HAdV3-Hexon-pMD18T and HAdV55-Hexon-pMD18T as well as genomic DNA of HAdV 3, HAdV 7, HAdV 21, and HAdV 55 with the HAdV-RPA method, respectively; 3, 4, 5, and 6 represent detection results of various concentrations of positive plasmids with HAdV3-RPA, HAdV7-RPA, HAdV21-RPA, and HAdV55-RPA methods, respectively; 8, 10, 12, and 14 represent detection results of various concentrations of genomic DNA with HAdV3-RPA, HAdV7-RPA, HAdV21-RPA, and HAdV55-RPA methods, respectively. The top and bottom red lines on each strip are the control and test lines, respectively. The concentrations of the templates are indicated. (−): negative result; (+): positive result.

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