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| Usage | Type | ID | Sequence (5-3) |
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| RPA-HAdV | Forward primer | HAdV-UniF | ACATCGCCGGACAGGATGCTTCGGAGTACC |
| Reverse primer | HAdV-UniR3/7 | Biotin-GGCCATGTCAAGCACTCTGTTGTCGCCTAC |
| Reverse primer | HAdV-UniR21/55 | Biotin-GGCCATATCCAGCACTCTGTTGTCGCCCAC |
| Probe | HAdV-UniP3/7 | FAM-GGTCTGGTGCAGTTCGCCCGTGCAACAGAC-[THF]-CCTACTTCAGTATGG-PO4 |
| Probe | HAdV-UniP21/55 | FAM-GGTCTGGTGCAGTTCGCCCGCGCCACAGAC-[THF]-CCTACTTCAATCTGG-PO4 |
| RPA-HAdV3 | Forward primer | HAdV3-F1 | ACCGGGAAGACAATACCTACTCTTACAAAG |
| Forward primer | HAdV3-F2 | ACGCTGGCTGTAGGCGACAACAGAGTGCTT |
| Forward primer | HAdV3-F3 | GCCATATTCCGGCACAGCTTACAATTCACT |
| Reverse primer | HAdV3-R1 | Biotin-TTCTTCTCCAACTTGAGGCTCTGGCTGATA |
| Reverse primer | HAdV3-R2 | Biotin-TTGTCTCCCTTCATGGAAGCAATGCCAAAT |
| Reverse primer | HAdV3-R3 | Biotin-GTAGCATGGCTTCATGTTGGTAGCTGGTTT |
| Probe | HAdV3-Probe | FAM-ATAGTTACAACGAATCGAGACAATGCAGTA-[THF]-CTACCACCACAAACA-PO4 |
| RPA-HAdV7 | Forward primer | HAdV7-F1 | TGACCACCGACCGTAGCCAGCGACTGATGC |
| Forward primer | HAdV7-F2 | GCCATATTCCGGCACAGCTTACAATTCACT |
| Forward primer | HAdV7-F3 | ATACATACTCTTACAAGTGCGGTACACCC |
| Reverse primer | HAdV7-R1 | Biotin-TTCTTCTCCAACTTGAGGCTCTGGCTGATA |
| Reverse primer | HAdV7-R2 | Biotin-TTCAACATCTCCTTCGGTTGGTGTTACTTT |
| Reverse primer | HAdV7-R3 | Biotin-CTACAAAGTTATCCCTGAAGCCAATGTAAT |
| Probe | HAdV7-Probe | FAM-TCTCAGTGGATAGTTACAACGGGAGAAGAC-[THF]-ATGCCACCACATACA-PO4 |
| RPA-HAdV21 | Forward primer | HAdV21-F1 | TTTGTGCCCGTTGACCGGGAAGACAATACC |
| Forward primer | HAdV21-F2 | AATACCTACGCATACAAAGTTCGATACACC |
| Forward primer | HAdV21-F3 | ATACAAAGTTCGATACACCTTGGCTGTGGG |
| Reverse primer | HAdV21-R1 | Biotin-TGCATAAATTGGTTTGGCTTCGCCGTCTGT |
| Reverse primer | HAdV21-R2 | Biotin-TCCCACCTGAGGTTCTGGTTGGTATAGTTT |
| Reverse primer | HAdV21-R3 | Biotin-GCTCTACCACCATACTTCTCAGTTGTTCCA |
| Probe | HAdV21-Probe | FAM-AGTGGATTGCTGAAGGCGTAAAAAAAGAAG-[THF]-TGGGGGATCTGACGA-PO4 |
| RPA-HAdV55 | Forward primer | HAdV55-F1 | GCTCCTAAAGGCGCTCCAAATACATCTCAG |
| Forward primer | HAdV55-F2 | TCAAACCCTATTCTGGTACGGCTTACAACT |
| Forward primer | HAdV55-F3 | CCGTTGACCGGGAGGACAATACATACTCTT |
| Reverse primer | HAdV55-R1 | Biotin-CGACTTTCTGATTTGGCTGCTCCGTTGTTT |
| Reverse primer | HAdV55-R2 | Biotin-CCATCAAGGTCAGTCCAAGTTTCATCTCCC |
| Reverse primer | HAdV55-R3 | Biotin-AACTTTCAAACCTATTGGGAGTCCTTCTTT |
| Probe | HAdV55-Probe | FAM-CGCGTAACAGAAGAGGAAAACAATACTACT-[THF]-CTTACACTTTTGGCA-PO4 |
| PCR for HAdV 3 (2835 bp) | Forward primer | Hexon3-F | CGAGGCTGAGTTGCTTTCA |
| Reverse primer | Hexon3-R | TCGGACGATGGCTTTGAG |
| PCR for HAdV 7 (2814 bp) | Forward primer | Hexon7-F | CGAGGCTGAGTTGCTTTCA |
| Reverse primer | Hexon7-R | TCGGACGATGGCTTTGAG |
| PCR for HAdV 55 (2847 bp) | Forward primer | Hexon55-F | CGACGCTGAGTTACTTTCA |
| Reverse primer | Hexon55-R | TTGGACAATGGCTCTGAG |
| Quantitative PCR | Forward primer | qPCR-F | ATGGCCACCCCATCGAT |
| Reverse primer | qPCR-R | ACTCAGGTACTCCGAAGCATCCT |
| Probe | qPCR-P | FAM-TGGGCATACATGCACATCGCCG-BHQ1 |
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