Research Article
Development of Rapid and Visual Nucleic Acid Detection Methods towards Four Serotypes of Human Adenovirus Species B Based on RPA-LF Test
Table 2
Sensitivity and specificity analysis of the established methods.
| | Samples | Methods | RPA-HAdV3 | RPA-HAdV7 | RPA-HAdV21 | RPA-HAdV55 | RPA-HAdV |
| | HAdV 3 positive () | Positive | 15 | 0 | 0 | 0 | 14 | | Negative | 0 | 15 | 15 | 15 | 1 | | Sensitivity | 100% | — | — | — | 93% | | Specificity | — | 100% | 100% | 100% | — | | HAdV 7 positive () | Positive | 2 | 82 | 1 | 1 | 81 | | Negative | 87 | 7 | 88 | 88 | 8 | | Sensitivity | — | 92% | — | — | 91% | | Specificity | 98% | — | 99% | 99% | — | | HAdV 21 positive () | Positive | 0 | 0 | 10 | 0 | 10 | | Negative | 10 | 10 | 0 | 10 | 0 | | Sensitivity | — | — | 100% | — | 100% | | Specificity | 100% | 100% | — | 100% | — | | HAdV 55 positive () | Positive | 0 | 0 | 0 | 17 | 17 | | Negative | 17 | 17 | 17 | 0 | 0 | | Sensitivity | — | — | — | 100% | 100% | | Specificity | 100% | 100% | 100% | — | — | | Influenza A (H1) positive () | Positive | — | — | — | — | 0 | | Negative | — | — | — | — | 41 | | Sensitivity | — | — | — | — | — | | Specificity | — | — | — | — | 100% | | Influenza A (H3) positive () | Positive | — | — | — | — | 38 | | Negative | — | — | — | — | 0 | | Sensitivity | — | — | — | — | — | | Specificity | — | — | — | — | 100% | | Overall | Sensitivity (95% confidence interval) | 100% (0.75-1, ) | 92% (0.84-0.97, ) | 100% (0.66-1, ) | 100% (0.77-1, ) | 93% (87%-97%, ) | | Specificity (95% confidence interval) | 98% (0.93-1)
() | 100% (0.90-1)
() | 99% (0.95-1)
() | 99% (0.94-1)
() | 100% (0.94-1)
() |
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