hsa_circ_0023305 Enhances Laryngeal Squamous Cell Carcinoma Progression and Modulates TRPM7 via miR-218-5p Sponging

Figure 5

Overexpression of TRPM7 or inhibition of miR-218-5p reverses the effect of hsa_circ_0023305 silencing on LSCC cell proliferation and invasion. RT-qPCR detection showing the expression of hsa_circ_0023305 (a, b), miR-218-5p (c, d) in Hep-2, and AMC-HN-8 cells. Data are presented as the ; vs. sh-NC; ^### vs. sh-hsa_circ_0023305. (e, f) RT-qPCR and Western blot detection showing the expression of TRPM7 in Hep-2 and AMC-HN-8 cells. Data are presented as the ; and vs. sh-NC; ^## and ^### vs. sh-hsa_circ_0023305. (g, h) CCK8 analysis showed the proliferative ability of Hep-2 and AMC-HN-8 cells. Data are expressed as the . and . Transwell detection showed the migration (i–k) and invasion (l–n) capacities of Hep-2 and AMC-HN-8 cells. Data are presented as the ; vs. sh-NC; ^### vs. sh-hsa_circ_0023305.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)