[Retracted] Isovitexin Suppresses Stemness of Lung Cancer Stem-Like Cells through Blockage of MnSOD/CaMKII/AMPK Signaling and Glycolysis Inhibition

Figure 2

Knockdown of MnSOD inhibiting stemness and glycolysis of LCSLCs. The LCSLCs were infected with recombinant adenovirus packaging MnSOD shRNA (Ad-sh MnSOD) or GFP (Ad-GFP) or blank control (mock). MnSOD shRNA inhibited the sphere-forming rate (a) and colony-forming rate (b) of LCSCs. MnSOD shRNA reduced the migratory (c) and invasive (d) capability of LCSLCs. MnSOD shRNA decreased the expression of CD133, CD44, and ALDH1 (e); Nanog and Bmi1 (f); and MnSOD, p-AMPK, and p-CaMkII in LCSLCs and did not apparently affect the expression of CaMkII and AMPK (g). (h) Knockdown of MnSOD suppressed the glycolysis in LCSLCs. vs. mock or Ad-GFP ().

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