Impact of Adenosine Analogue, Adenosine-5-N-Ethyluronamide (NECA), on Insulin Signaling in Skeletal Muscle Cells

Table 1

List of gene primer and probe sequences.

Gene	Sequences (5-3)
NR4A1	Probe: 5-CTTTATCCTCCGCCTGGCCTACCGA-3; Forward primer: 5-TGTTGCTAGAGTCCGCCTTTC-3; Reverse primer: 5-CAGGCCTGAGCAGAAGATGAG-3
NR4A2	Probe: 5-TACGCTTAGCATACAGGTCCAACCCAGTG-3; Forward primer: 5-CCAAAGCCGATCAGGACCT-3; Reverse primer: 5-GACCACCCCATTGCAAAAGAT-3
NR4A3	Probe: 5-ACTGTCCCACCGACCAGGCCACT-3; Forward primer: 5-GACGCAACGCCCAGAGAC-3; Reverse primer: 5-TAGAACTGCTGCACGTGCTCA-3
PGC-1α	Probe: 5-TGGAACTCTCTGGAACTGCAGGCCTAACT-3; Forward primer: 5-TTCCCCATTTGAGAACAAGACTATT-3; Reverse primer: 5-GTTATCTTGGTTGGCTTTATGAGGA-3
CPT1B	Probe: 5-CCTACATGATCGCAGGCGAAAACACAA-3; Forward primer: 5-GGCCGACCACGGATACG-3; Reverse primer: 5-ACTCGATAACTTGCTGGAAACATG-3
ACCβ	Probe: 5-ATCGAGACGGTGCTCATCGCCAAT-3; Forward primer: 5-TGTCACCCGCTTTGGAGG-3; Reverse primer: 5-CATACACTTGACCGCAGCGAT-3
LCAD	Probe: 5-GGAATGAAAGCCCAGGACACAGCAGAA-3; Forward primer: 5-GGTGGAGAATGGAATGAAAGGAT-3; Reverse primer: 5-GCACTAGCTGGCAATCGAACA-3
PDK4	Probe: 5-CGTCGCCAGAATTAAAGCTCACACAAGTC-3; Forward primer: 5-AGCAGTAGTCGAAGATGCCTTTG-3; Reverse primer: 5-ATGTGGTGAAGGTGTGAAGGAA-3
HKII	Probe: 5-AGTTCCTGTCTCAGATAGAGAGCGACTGCCT-3; Forward primer: 5-GCATCTCAGAGCGCCTCAAG-3; Reverse primer: 5-GATGGCACGAACCTGTAGCA-3
PFK	Probe: 5-CTGCCCTGCACCGCATTGTAGAGATC-3; Forward primer-5-TGGCACTGATATGACCATTGGT-3; Reverse primer-5-TGAGCGGTGGTGGTGATG-3
IL-6	Probe: 5-CTCTCCGCAAGAGACTTCCAGCCAGTT-3; Forward primer: 5-GCCCTTCAGGAACAGCTATGA-3; Reverse primer: 5-TGTCAACAACATCAGTCCCAAGA-3
TATA-BOX	Probe: 5-TCCCAAGCGGTTTGCTGCAGTCA-3; Forward primer: 5-TTCGTGCCAGAAATGCTGAA-3; Reverse primer: 5-GTTCGTGGCTCTCTTATTCTCATG-3