[Retracted] Hydroxyphenyl Butanone Induces Cell Cycle Arrest through Inhibition of GSK3β in Colorectal Cancer

Figure 3

The effect of HCT116 and LOVO cells in colorectal cancer downregulated by GSK3β expression and β-catenin raised by the chemical extract of red raspberry on expression and localization in cells and Wnt pathway. (a) GSK3β expression downregulated RT-PCR detection. The expression level of GSK3β mRNA in the HCT116 transfected group was of that in the control group and that in the LOVO transfected group was of that in the control group by RT-PCR detection. (b) Western blot detection of downregulated levels of GSK3β expression. GSK3β mRNA expression level in the HCT116 transfected group was 29% of that in the control group by Western blot analysis and was 41% in the LOVO transfected group of that in the control group. (c) Under the same experimental conditions, effects of chemical extracts of raspberry with different concentrations and raspberry ketone on the Wnt pathway of colorectal cancer HCT116 and LOVO cells with Si-RNA transfection downregulated GSK3β expression. (d) Under the same experimental conditions, effects of different concentrations of red raspberry phytochemical extracts and raspberry ketone on the expression and localization in cells of β-catenin in Si-RNA transfection colorectal cancer HCT116 and LOVO cells downregulated by GSK3β expression. .

(a)

(b)

(c)

(d)