Curcumin Alleviates Palmitic Acid-Induced LOX-1 Upregulation by Suppressing Endoplasmic Reticulum Stress in HUVECs

Figure 3

Effects of PA on LOX-1 and ER stress marker expression in HUVECs. (a) The protein expression level of LOX-1 was measured by Western blotting. (b) The expression of LOX-1 was quantified via densitometry using ImageJ software (; 1-way ANOVA with Duncan’s posthoc test). (c) The mRNA expression level of LOX-1 was measured via quantitative PCR (; 1-way ANOVA with Duncan’s posthoc test). (d) The protein expression levels of Bip and CHOP were measured by Western blotting. The expression of Bip (e) and CHOP (f) was quantified via densitometry using ImageJ software (; 1-way ANOVA with Duncan’s posthoc test). (g–i) The mRNA expression levels of ER stress-related genes (Bip, CHOP, and XBP1s) were measured using quantitative PCR (; 1-way ANOVA with Duncan’s posthoc test). The data are shown as (, , vs. the Cont group).

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