Homeobox B9 Promotes the Progression of Hepatocellular Carcinoma via TGF-β1/Smad and ERK1/2 Signaling Pathways

Figure 1

The expression profile of HOXB9 in HCC tissues. (A) Immunohistochemistry was performed to measure the expression of HOBX9 in HCC and liver tissues (200× magnification for the whole picture and 400× magnification for that at top right corner); (a) HOBX9 in nontumor tissues; (b) low HOBX9 expression in HCC tissues; (c) moderate HOBX9 expression in HCC tissues; (d) high HOBX9 expression in HCC tissues; (e–f) negative control for nontumor tissues and HCC tissues, respectively. (B) Semiquantitative analysis of HOBX9 by Immunohistochemistry in tissues from HCC tumor and nontumor. (C) RT-qPCR was used to detect the HOXB9 mRNA levels in four groups. (D) Western blot was used to detect the HOXB9 protein levels in four groups. (E) The present survival was determined in HCC patients who had HOBX9 high () and low expression (). vs. nontumor.

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