Homeobox B9 Promotes the Progression of Hepatocellular Carcinoma via TGF-β1/Smad and ERK1/2 Signaling Pathways

Figure 3

Expression prolife of HOBX9, TGF-β1 pathway and MAPK pathway in HCC cell lines. (a) RT-qPCR analysis of HOXB9 expression in immortalized human live cell (THLE3) and HCC cell lines (including BEL-7402, BEL-7404, BEL-7405, HepG2, Hep3B, and SNU475). (b–c) Western blot was used to detect the expression of TGF-β1 signaling pathway (including Smad7, Smurf2, TGF-β1, and p-Smad2) in THLE3 cells and HCC cell lines. (d–e) Western blot was used to detect the expression of MAPK signaling pathway (including ERK1/2, p-38, and JNK pathway) in THLE3 cells and HCC cell lines. vs. THLE3.

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