WT1 Inhibits Human Renal Carcinoma Cell Proliferation and Induces G2/M Arrest by Upregulating IL-24 Expression

Figure 3

WT1 overexpression inhibits the proliferation of A498 cells by promoting G2/M arrest. WT1 levels in renal cancer cell lines from the CCLE database (a). WT1 mRNA levels measured by RT-qPCR (b). Cells were transfected with WT1 or control vectors (2 μg/well), and WT1 levels were measured by RT-qPCR (c) or Western blotting (d). A498-Luc2 cells transfected with the WT1 vector or empty vector (0.2 μg/well). FLUX measurements were captured (e). For normalization of luciferase activity, the luciferase signal per well of the control cells was set to 1. Quantitative data represent the ( per group) (f). Cell cycle analysis of WT1-overexpressing A498 cells by flow cytometry. Flow cytometry of WT1-overexpressing A498 cells showing G2/M arrest was assessed via flow cytometry. Histograms of cell cycle distribution (g). Statistical diagram of the cell cycle distribution (h). Experiments were conducted in triplicate (); error bars represent standard errors; .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)