Research Article
WT1 Inhibits Human Renal Carcinoma Cell Proliferation and Induces G2/M Arrest by Upregulating IL-24 Expression
Figure 5
WT1 overexpression can induce G2/M arrest by upregulating IL-24 expression in RCC cells. The expression of (a) WT1 or (b) IL-24 mRNA in A498 cells transfected with the WT1 vector was detected by RT-qPCR. (c) Standard curve for ELISA-based detection of IL-24 levels. (d) The expression of IL-24 protein in A498 cells transfected with the WT1 vector was detected by ELISA. (e) IL-24 levels in IL-24-overexpressing cells detected by ELISA. Flow cytometry of IL-24-overexpressing A498 cells showing G2/M arrest was assessed via flow cytometry. (f) Histograms of cell cycle distribution. (g) Statistical diagram of the cell cycle distribution. Experiments were conducted in triplicate (); error bars represent standard errors; .
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