6-O-Galloylpaeoniflorin Exerts Inhibitory Bioactivities in Human Neuroblastoma Cells via Modulating AMPK/miR-489/XIAP Pathway

Figure 3

GPF treatment increases the expression of miR-489 in NBM cells. (a) SH-SY5Y cells were treated with the indicated concentrations of GPF. At 6 h after GFP treatment, phosphorylated AMPK in cells was detected by immunoblotting. (b) SH-SY5Y cells were treated with the indicated concentrations of GPF for 24 h or 100 μM GPF for different durations. The expression of miR-489 was determined by qPCR. (c) After pretreatment with or without compound C for 6 h, SH-SY5Y cells were exposed to GPF treatment for another 18 h, and the expression of miR-489 was determined by qPCR. ; .

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