Phylogenicity and Virulence Profiles of Clinical Escherichia coli Isolates in the Ho Teaching Hospital of Ghana

Table 2

Oligonucleotide primers used to detect virulence genes.

Target gene	Primer ID	Primer sequence (5-3)	Amplicon size (bp)	Reference
ibeA	ibeA_F	TGAACGTTTCGGTTGTTTTG	814	[17]
	ibeA_R	TGTTCAAATCCTGGCTGGAA
hlyA	hylA_F	AACAAGGATAAGCACTGTTCTGGC	1177	[17]
	hylA_R	ACCATATAAGCGGTCATTCCCGTCA
hlyF	hylF_F	TCGTTTAGGGTGCTTACCTTCAAC	444	[17]
	hylF_R	TTTGGCGGTTTAGGCATTCC
neuC	neuC_F	AGGTGAAAAGCCTGGTAGTGTG	676	[17]
	neuC_R	GGTGGTACATCCCGGGATGTC
iutA	iutA_F	ATGAGCATATCTCCGGACG	587	[17]
	iutA_R	CAGGTCGAAGAACATCTGG
sfa/foc	sfa/foc_F	CTCCGGAGAACTGGGTGCATCTTAC	410	[17]
	sfa/foc_R	CGGAGGAGTAATTACAAACCTGGCA
fimA	fimA_F	CGGCTCTGTCCCTSAGT	500	[17]
	fimA_R	GTCGCATCCGCATTAGC
yfcV	yfcV_F	ACATGGAGACCACGTTCACC	292	[18]
	yfcV_R	GTAATCTGGAATGTGGTCAGG
cnf 1	cnf 1_F	AAGATGGAGTTTCCTATGCAGGAG	498	[17]
	cnf 1_R	CATTCAGAGTCCTGCCCTCATTATT
vat	vat_F	TCAGGACACGTTCAGGCATTCAGT	1100	[18]
	Vat_R	GGCCAGAACATTTGCTCCCTTGTT
fyuA	fyuA_F	F_GTAAACAATCTTCCCGCTCGGCAT	850	[18]
	fyuA_R	R_TGACGATTAACGAACCGGAAGGGA