Nanohydroxyapatite-Blasted Bioactive Surface Drives Shear-Stressed Endothelial Cell Growth and Angiogenesis

Figure 3

HAnano® upmodulates EC phenotype-related genes. Shear-stressed endothelial cell were subjected to Ti-enriched medium for 24 hours, and the samples were harvested to allow gene expression using RT-qPCR methodology. Our data shows there is a significant higher expression of VEGF (a) and eNOS (b) genes in response to HAnano®, as well as AKT gene (c). The graphs bring the -fold change of the profile of transcripts normalized to the 18S gene (housekeeping gene). Significant differences were considered when , represented by when compared to the Ctrl group and by ^# when compared to the W/DAE group.

(a)

(b)

(c)