Nanohydroxyapatite-Blasted Bioactive Surface Drives Shear-Stressed Endothelial Cell Growth and Angiogenesis

Figure 6

CDK4 is upmodulated in response to HAnano®. Thereafter, understanding its relevance on endothelial cells migration and angiogenesis, we postulated the hypothesis that HAnano® modulates cell cycle-related genes. Although MAPKs p38 (a) and ERK (b) genes were downregulated in response to HAnano®, CDK4 gene expression was significantly higher ((c) approximately 2.5-fold changes), while CDK2 gene was downregulated (d). Importantly, cell cycle suppressor genes, p15 (e) and p21 (f), were downregulated in response to both W/DAE and HAnano®. The graphs bring the -fold change of the profile of transcripts normalized to the 18S gene (housekeeping gene). Significant differences were considered when p <0.05, represented by when compared with the Ctrl group and by ^# when compared to the W/DAE group.

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