Nanohydroxyapatite-Blasted Bioactive Surface Drives Shear-Stressed Endothelial Cell Growth and Angiogenesis

Table 1

Expression primers sequences and qPCR cycle conditions.

Gene	Primer	5-3 sequence	Work condition
AKT	Forward 1	CAGCGCGGCCCGAAGGAC	95°C -3 s, 55°C -8 s, 72°C -20s
	Forward 2	GGACTCCCGTTTGCGCCAGT
	Reverse	GACGCTCACGCGCTCCTCTC
CFL1	Forward	TGTGCGGCTCCTACTAAACG	95°C -3 s, 55°C -8 s, 72°C -20s
	Reverse	TCCTTGACCTCCTCGTAGCA
P15	Forward	GGGACTAGTGGAGAAGGTGC	95°C -3 s, 55°C -8 s, 72°C -20s
	Reverse	CATCATCATGACCTGGATCGC
P21	Forward	GCTGCCGAAGTCAGTTCCTT	95°C -3 s, 55°C -8 s, 72°C -20s
	Reverse	ATCTGTCATGCTGGTCTGCC
CDK2	Forward	CTTTGCTGAGATGGTGACTCG	95°C -3 s, 55°C -8 s, 72°C -20s
	Reverse	GCCTCCCAGATTCCTCATGC
CDK4	Forward	CTCTCTAGCTTGCGGCCTG	95°C -3 s, 55°C -8 s, 72°C -20s
	Reverse	GCAGGGATACATCTCGAGGC
VEGF	Forward	TGCAGATTATGCGGATCAAACC	95°C -3 s, 55°C -8 s, 72°C -20s
	Reverse	TGCATTCACATTTGTTGTGCTGTAG
ENOS	Forward	TATTTGATGCTCGGGACTGC	95°C -3 s, 55°C -8 s, 72°C -20s
	Reverse	AAGATTGCCTCGGTTTGTTG
P38	Forward	GAGAACTGCGGTTACTTA	95°C -3 s, 55°C -8 s, 72°C -20s
	Reverse	ATGGGTCACCAGATACACAT
ERK	Forward 1	AACAGGCTCTGGCCCACCCAT	95°C -3 s, 55°C -8 s, 72°C -20s
	Forward 2	CGCCCCTCCAAACGGCTCAA
	Reverse	GCAGCGCCTCCCTTGCTAGA
ITGB1	Forward	GCCGCGCGGAAAAGATGAA	95°C -3 s, 55°C -8 s, 72°C -20s
	Reverse	TGCTGTTCCTTTGCTACGGT
FAK	Forward	TCAGCTCAGCACAATCCTGG	95°C -3 s, 55°C -8 s, 72°C -20s
	Reverse	CTGAAGCTTGACACCCTCGT
SRC	Forward	CAACACAGAGGGAGACTGGT	95°C -3 s, 55°C -8 s, 72°C -20s
	Reverse	AGCTTCTTCATGACCTGGGC
18S	Forward	CGGACAGGATTGACAGATTGATAGC	95°C -3 s, 55°C -8 s, 72°C -20s
	Reverse	TGCCAGAGTCTCGTTCGTTATCG