Research Article
Pioglitazone Mediates Cardiac Progenitor Formation through Increasing ROS Levels
Table 1
Primer sequences used for gene expression analysis.
| | Genes | Primer sequence (5-3) | Annealing temp (°C) | Accession no. |
| | Gapdh | F: TGCCGCCTGGAGAAACC
R: TGAAGTCGCAGGAGACAACC | 58 | NM_008084.2 | | PPARγ | F: TGAGACCAACAGCCTGAC
R: GTTCACCGCTTCTTTCAAATC | 60 | NM_001127330.1 | | Nkx2.5 | F: TTAGGAGAAGGGCGATGAC
R: AGGGTGGGTGTGAAATCTG | 57 | NM_008700.2 | | Mef2c | F: CGAGTGTAAGTGTCTAATG
R: CCTATTGTCAGAATTGCTAT | 54 | NM_001170537.1 | | α-MHC | F: CAGAAGCCTCGCAATGTC
R: CGGTATCAGCAGAAGCATAG | 58 | NM_001164171.1 | | cTnT | F: ACAGAGGAGGCCAACGTAGAA
R: CTCTCTCCATCGGGGATTCTT | 60 | NM_001130181.2 |
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