USP2 Inhibits Lung Cancer Pathogenesis by Reducing ARID2 Protein Degradation via Ubiquitination

Figure 4

USP2 inhibits lung cancer cell migration and invasion in vitro; H1299 cells were transfected with Sh USP2#1, Sh USP2#2, or negative control vector. A549 cells were transfected with USP2 overexpression vector or negative control vector. (a) Transwell study on H1299/A549 cells transfected with USP2 shRNAs or USP2 overexpression vectors. Relative numbers of migrated cells were calculated in the right chart ( indicates compared with negative control vector). (b) Invasion study on H1299/A549 cells transfected with USP2 shRNAs or USP2 overexpression vectors. Relative numbers of invaded cells were calculated in the right chart ( indicates compared with negative control vector). (c–e) Tumor cell EMT markers (MMP9, E-Cadherin, N-Cadherin, and Vimentin) and USP2 protein expression value evaluation in H1299 and A549 cell lines transfected with shRNAs or overexpression vectors.

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