USP2 Inhibits Lung Cancer Pathogenesis by Reducing ARID2 Protein Degradation via Ubiquitination

Figure 5

USP2 affects lung cancer cell migration and invasion through ARID2 regulation; H1299 cells were transfected with SiARID2#1, SiARID2#2, SiARID2#3, or negative control vector. A549 cells were transfected with ARID2 overexpression vector or negative control vector. (a, b) Design and validation of ARID2-specific siRNAs and overexpression vectors’ effects on the mRNA and protein expression of ARID2 in H1299 and A549 cells ( indicates compared with negative control vector). (c, d) Transwell study on A549 cells transfected with ARID2 siRNA in combination with or without USP2 overexpression vectors. Relative numbers of migrated cells were statistically compared ( indicates compared with USP2 overexpression vector or negative control vector). (e, f) Tumor cell invasion assay on A549 cells transfected with ARID2 siRNA in combination with or without USP2 overexpression vectors. Relative numbers of invaded cells were statistically compared ( indicates compared with USP2 overexpression vector or negative control vector). (g, h) Wound healing assay on H1299 cell line groups transfected with USP2 shRNAs in combination with or without ARID2 overexpression vectors. Relative numbers of migrative cells were statistically compared ( indicates ). (i) Tumor cell EMT markers (MMP9, E-Cadherin, N-Cadherin, and Vimentin) and USP2 protein expression value evaluation in H1299 cell lines groups transfected with USP2 shRNAs in combination with or without ARID2 overexpression vectors.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)