Neural Peptide α-CGRP Coregulated Angiogenesis and Osteogenesis via Promoting the Cross-Talk between Mesenchymal Stem Cells and Endothelial Cells

Figure 1

(a) Cell viability assay by CCK-8 assay for MSC proliferation in different concentrations of CGRP for 2 days (). (b) cAMP levels were released by MSCs cultured in different concentrations of CGRP for 2 days. (c) ALP staining of MSCs in different groups after culture for 4 and 7 days, respectively (scale bar: 250 μm) and (e) ALP activity of MSCs cultured in different groups after culture for 4 and 7 days (). (d) Representative ARS stained the mineral deposition (red, red arrows) of MSCs after culture for 14 days (scale bar: 250 μm). (f) Quantification of MSCs mineralization () from different groups after culture for 14 days. , ^#.

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