Neural Peptide α-CGRP Coregulated Angiogenesis and Osteogenesis via Promoting the Cross-Talk between Mesenchymal Stem Cells and Endothelial Cells

Table 1

Real-time PCR primers used in this study.

Target gene	Genebank (accession no.)	Primers	Product size (bp)
β-Actin	NM_031144.2	GGAGATTACTGCCCTOGCTCCTA GACTCATCGTACTCCTGCTTGCTG	150
Runx2	NM_053470.2	GCCGTAGAGAGCAGGGAAGAC CTGGCTTGGATTAGGGAGTCAC	150
ALP	NM_013059	AGCGACACGGACAAGAAGC GGCAAAGACCGCCACATC	183
Col I	NM_053304.1	CCTGAGCCAGCAGATTGA; TCCGCTCTTCCAGTCAG	106
OCN	M11777	GAGGGCAGTAAGGTGGTGAA CGTCCTGGAAGCCAATGTG	154
OPN	M99252	GACAGCAACGGGAAGACC; CAGGCTGGCTTTGGAACT	216
OPG	RUN94330	GCCCAGACGAGATTGAGAG; CAGACTGTGGGTGACGGTT	173
bFGF	NM_001361665.2	TCCACCTATAATTGGTCAAAGTGGT; CATCAGTTACCAGCTCCCCC	121
EGF	NM_001963.6	TGGTGATGGGAGGATGACTTG; GGCCAGTGACTCAGCAGAAA	158
HIF-1a	AB733094.1	AGCTTGCTCATCAGTTGCCA; CCAGTTAGTTCAAACAGCATCCA	125
VEGF-a	BC065522.1	CCCACTGAGGAGTCCAACATC; CGGCTTGTCACATTTTTCTTGTC	150