Research Article
M2 Macrophage-Derived Exosomes Inhibit Apoptosis of HUVEC Cell through Regulating miR-221-3p Expression
Figure 3
Construction of HUVEC oxidative stress and inflammatory injury models. A: HUVECs were cultured with 100 mg/L oxidized low-density lipoprotein (ox-LDL) for 24 h to establish an oxidative stress injury model. Cell proliferation was detected by CCK8 assays, and cell apoptosis was determined using Annexin V-FITC/PI staining followed by flow cytometry (). B: HUVECs were cultured with 50 ng/mL tumor necrosis factor-α (TNF-α) for 24 h to establish an inflammatory injury model, Cell proliferation was measured by CCK8 assay, and cell apoptosis assay was determined with Annexin V-FITC/PI staining followed by flow cytometry analysis (). compared with Normal group; .
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