M2 Macrophage-Derived Exosomes Inhibit Apoptosis of HUVEC Cell through Regulating miR-221-3p Expression

Figure 4

M2 macrophages enhanced HUVEC cell proliferation and inhibited HUVEC cell apoptosis in the HUVEC oxidative stress injury model. (a, c) Cell apoptosis assays were performed using Annexin V-FITC/PI staining followed by flow cytometry (). (b) Cell proliferation was determined using CCK8 assays. HUVECs were treated with or without M0, M1, or M2 macrophages (). (d) qRT-PCR analysis of mRNA levels of inflammatory factors IL-6, IL-10, IL-1β, and TNF-α mRNA in HUVECs (). (e) Representative western blots of apoptosis-related protein (Caspase-3, Bcl2, and c-myc) expressions in HUVECs after macrophage stimulation (). Compared with control group. , , .

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