M2 Macrophage-Derived Exosomes Inhibit Apoptosis of HUVEC Cell through Regulating miR-221-3p Expression

Figure 8

Effect of miR-221-3p in the HUVEC oxidative stress injury model. (a) The relative miRNA expression of miR-4449, miR-211-5p, miR-10b-3p, miR-503-5p, miR-708-5p, and miR-221-3p in macrophages and macrophage Exos (). (b) The relative miRNA expression of miR-221-3p after transfection (). (c, e) Cell apoptosis assay was determined using Annexin V-FITC/PI staining followed by flow cytometry (). (d) Cell proliferation was determined by CCK8 assay (). (f) qRT-PCR analyses of inflammatory factors IL-6, IL-10, IL-1β, and TNF-α mRNA in HUVECs (). (g) Representative western blots of apoptosis-related proteins (Caspase-3, Bcl2, and c-myc) and Grb10 protein expression in HUVEC cells after liposome transfection (). Compared with control group; , , .

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