Laser Speckle Flowmetry for the Prognostic Estimation Study of Permanent Focal Ischemia in Mice

Figure 1

Schematic diagram of the laser speckle imaging system. (a) Schematic diagram of the RFLSI III laser speckle perfusion imager. Speckle images are acquired by the laser at 785 nm. Scattered laser light from the brain is captured by a CMSO camera, followed by digitization by a computer-based frame grabber. (b) Representative images of cerebral blood flow. Left image: real picture of the mouse brain. Middle image: laser speckle contrast imaging of cerebral blood flow. Right image: pseudocolor image of the middle image. Each image covers an area of , corresponding to pixels.

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