Repression of the Antioxidant Pyrroloquinoline Quinone in Skin Aging Induced by Bmi-1 Deficiency

Figure 2

The effect of PQQ on skin morphology of Bmi-1^-/- mice. (a) Representative micrographs of paraffin embedded skin sections from WT, Bmi-1^-/-, and Bmi-1^-/-+PQQ mice stained for HE, (c) elastic fiber, (e) total collagen, and (g) immunohistochemically for type I collagen. (b) The skin thickness, the percentage of (d) elastic fiber-positive, (f) total collagen-positive, and (h) type I collagen-positive area were calculated and presented using image analysis. Values are shown as the of seven determinations per group. , , and versus WT mice; ^& and ^&& versus Bmi-1^-/- mice.

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