[Retracted] LRSAM1 E3 Ubiquitin Ligase Promotes Choriocarcinoma Progression and Metastasis via p53/p21 Signaling Impediment

Figure 1

LRSAM1 expression is upregulated, and LRSAM1 knockdown inhibits cell proliferation in choriocarcinoma. (a) LRSAM1 overexpression is observed in choriocarcinoma patients from the Human Protein Atlas. (b) Significant LRSAM1 upregulation in JEG-3 and JAR choriocarcinoma cellular structures versus HTR8/sev8 cellular structures. (c) RT-qPCR assay reveals the successful knockdown of the LRSAM1 gene. (d) The CCK-8 assay shows restricted cell vivacity in JEG-3 and JAR cellular structures versus HTR8/sev8 cellular structures. (e) Clone formation assay reveals hindered proliferating in JEG-3 and JAR cellular structures versus HTR8/sev8 cellular structures. (f) FACS analysis reveals G1 arrest that is only detectable in the si-LRSAM1 group. (g) Western blot shows lower expressions of cell cycle-related protein contents, Cyclin D1 and Ki-67, following LRSAM1 knockdown.

(a)

(b)

(c)

(d)

(e)

(f)

(g)