[Retracted] LRSAM1 E3 Ubiquitin Ligase Promotes Choriocarcinoma Progression and Metastasis via p53/p21 Signaling Impediment

Figure 5

LRSAM1 knockdown inhibits choriocarcinoma cell proliferation and metastasis via p53/p21 signaling activation. (a) The RT-qPCR assay shows significantly increased P53 and P21 mRNA expressions in the si-LRSAM1 group versus si-NC. (b) Western blot results of p21, p53, and p-p53 protein in the control, si-LRSAM1, and si-LRSAM1+PFT group, in JEG-3 and JAR cellular structures. (c) PFT treatment help restore cell proliferating in transfected cellular structures, as indicated by colon formation analysis. (d–f) Transwell migratory flow and Matrigel adhesion assays reveal that PFT treatment improves cell migratory flow, invasive force, and adhesion in transfected cellular structures versus untreated controls.

(a)

(b)

(c)

(d)

(e)

(f)