The Leaf Extract of Mitrephora chulabhorniana Suppresses Migration and Invasion and Induces Human Cervical Cancer Cell Apoptosis through Caspase-Dependent Pathway

Figure 3

Effects of the leaf extract of MC on the cell morphological changes and nuclear fragmentation of HeLa cervical cancer cells and 3T3 fibroblast cells. (a) HeLa cells were treated with 125 μg/mL of MC extract for 24 h. (b) 3T3 fibroblast cells were treated with 125 μg/mL of MC extract for 24 h. Micrographs were taken at 0 and 24 h by a bright-field microscope (phase-contrast) and a fluorescent microscope to observe DNA fragmentation stained by DAPI (blue). Yellow arrow indicates DNA fragmentation, and the indicated cell is magnified in the right corner with yellow border. Data are representatives of three individual replicates.