Research Article
The Leaf Extract of Mitrephora chulabhorniana Suppresses Migration and Invasion and Induces Human Cervical Cancer Cell Apoptosis through Caspase-Dependent Pathway
Figure 5
Effects of MC extract on stimulating caspase-dependent death signaling pathway. (a) HeLa cells were treated with MC extract at different concentrations (UT: untreated; 62.5, 125, and 250 μg/mL) for 24 h, subjected to Annexin V/PI staining and flow cytometry. (b) Quantitative analysis of apoptotic HeLa cells from Annexin V/PI staining and flow cytometry. (c) Full-length and cleaved caspase 9, caspase 7, and PARP were detected by western blot analysis. Actin was used as an internal control. Data are representatives of three individual replicates.
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