[Retracted] Kukoamine A Improves Mycoplasma pneumoniae Pneumonia by Regulating miR-222-3p/Superoxide Dismutase 2

Figure 2

miR-222-3p is highly expressed in exosomes and induces inflammation in human alveolar basal epithelial cells. (a) Exosomes extracted from serum samples from MPP patients and normal healthy controls, and then RT-qPCR was used for detecting the expression levels of miR-222-3p (; vs. control). (b)–(f) A549 cells were treated with MPP serum exosomes (100 μg/mL) for 6, 12, and 24 h. (b, c) RT-qPCR was used to detect the mRNA expression levels of miR-222-3p (b) and SOD2 (c). (d) Western blot was used to detect the protein expression levels of SOD2 and NF-κB. (e, f) at 6, 12, and 24 h, the expression of IL-6 (e) and TNF-α (f) in the cell supernatant was detected by ELISA. , , vs. 0 h; ^#, ^## vs. 6 h; ⁺, ⁺⁺ vs. 12 h.

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