[Retracted] Kukoamine A Improves Mycoplasma pneumoniae Pneumonia by Regulating miR-222-3p/Superoxide Dismutase 2

Figure 3

Mechanisms of miR-222-3p repression of SOD2 transcription in human alveolar basal epithelial cells. (a)–(c) miR-222-3p inhibitors or mimics were transfected into A549 cells. (a) The miRNA expression of miR-222-3p was detected by RT-qPCR. , vs. NC (negative control of mimics or inhibitors). (b, c) The mRNA (b) and protein (c) expression of the endogenous expression of SOD2 in A549 cells was detected by RT-qPCR. , vs. NC. (d) Assay of luciferase activities in A549 cells transfected with wild-type (WT) or mutant (Mut) constructs along with miR-222-3p mimics or inhibitors as indicated. , vs. . (e)–(g) miR-222-3p mimics and SOD2 overexpression vectors were concomitantly or individually transfected into cells A549 cells. (e, f) ELISA for detecting the expression levels of IL-6 (e) and TNF-α (f) in the cell supernatant. (g) The protein expression of SOD2 and NF-κB was detected by western blot. , vs. NC; ^##, ^### vs. .

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