[Retracted] Kukoamine A Improves Mycoplasma pneumoniae Pneumonia by Regulating miR-222-3p/Superoxide Dismutase 2

Figure 4

KuA attenuates miR-222-3p mimic-induced inflammation by targeting SOD2. (a, b) A549 cells were treated with MPP serum exosomes (100 μg/mL), followed by 24 h treatment of KuA at different concentrations (0, 5, 10, and 20 μM) of as indicated. (a) The mRNA expression of miR-222-3p was detected by RT-qPCR. (b) The protein expression of SOD2 and NF-κB (cytoplasm and nuclear) was detected by western blot. vs. control (A549 cells without treatment); ^#, ^##, ^### vs. ; ⁺, ⁺⁺ vs. ; ^& vs. . (c)–(e) A549 cells were treated with miR-222-3p mimics, followed by treatment of KuA at 10 μM for 24 h. (c, d) ELISA to identify the levels of IL-6 (c) and TNF-α (d) in the cell supernatant. (e) The protein levels of SOD2 and NF-κB (cytoplasm and nuclear) were examined by western blot. , vs. control; ^#, ^## vs. .

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