MTA-Based Cements: Biocompatibility and Effects on the Gene Expression of Collagen Type 1 and TGF-β1

Figure 1

Micrographs of hFDP morphology at 24 h after application of the elutes of bioactive cements or fresh medium. Each cell culture was examined under an inverted optical microscope (Leica DM IL LED, Wetzlar, Germany) without any type of staining at a magnification of 40x. (a) Control group; (b) MTA-HP, shows alterations in cell membrane integrity (red arrow) an almost twofold increase in cell size and presence of intracytoplasmatic granules (yellow arrow); (c) Neo-MTA, increased presence of intracytoplasmatic granules, and the size of the cell is slightly increased (yellow arrow); (d) MTA-Ang, this treatment cell size is more conserved but decreases the amount of phyllopodia (red arrow) spindle cells with thin and branched cytoplasmic extensions were observed (green circle), alterations in membrane integrity and the presence of intracytoplasmatic granules.

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