MTA-Based Cements: Biocompatibility and Effects on the Gene Expression of Collagen Type 1 and TGF-β1

Figure 6

Ultrastructural analysis of fibroblasts with SEM. SEM micrographs of cultured fibroblasts in contact with the eluates of the bioactive cements for 24 h. 1000x. (a) Negative control group, fibroblast with a morphology and extensions in normal appearance. (b) MTA-HP group, cells are poorly dressed to the surface with a compact structure they were detached from the surface where they are growing. (c) Neo-MTA group, the cell has an adequate size; however, the shape and structure of the cell are altered as well as the number of focal contacts and phyllopodia that help the cell to adhere to the material on which they are growing are significantly reduced. (d) MTA-Ang group, the shape and structure of the cell are normal, robust focal contacts with a good generation of filopodias as seen in the magnification box. This micrograph highlights the focal contacts of the fibroblasts that represent a good adhesion to the surface that supports them; these characteristics can be extrapolated to a good response to the material to which it is exposed.

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