Bioactive Vitamin D Attenuates MED28-Mediated Cell Growth and Epithelial–Mesenchymal Transition in Human Colorectal Cancer Cells

Figure 2

MED28 knockdown mimicked the effect of calcitriol on E-cadherin and Wnt/β-catenin signaling in HT29 human colorectal cancer cells. HT29 cells were treated with calcitriol (100 nM) for 48 h (a) or undergone MED28-specific siRNA (siMED28) for 72 h (b) with respective controls and subjected to Western blotting with the antibodies indicated. The ratios below the representative images (left panels) indicate the relative expression of the specific proteins with respect to those of 0 nM (a) or siControl (b) after normalization with the expression of the corresponding loading controls, β-actin or α-tubulin. Densitometric data (right panels) are expressed as , ; , , , as compared with 0 nM (a) or siControl (b).

(a)

(b)