High-Calcium Microenvironment during the Development of Kidney Calculi Can Promote Phenotypic Transformation of NRK-52E Cells by Inhibiting the Expression of Stromal Interaction Molecule-1

Figure 3

(a and b) The NRK-52E cells were treated with the concentrations of high-calcium medium (0.7 mg/mL), and then, the expression of calcium channel protein (STIM1 and Orai1) and phenotypic marker proteins (E-cadherin and OPN) were determined by western blot. (c) The above results were further verified by RT-PCR analysis. (d and e) The adhesion and deposition of calcium salt on NRK-52E cells increased significantly, when the NRK-52E cells were treated with the concentrations of high-calcium medium (0.7 mg/mL).

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