T Lymphocyte Infiltration in Association with IDO1 Expression in Resected Lung Adenocarcinoma and Normal Adjacent Lung Tissues

Figure 2

Protein and mRNA level of IDO1 in the lung adenocarcinoma and adjacent normal tissues. (a) Representative image data of IDO1. Protein level of IDO1 in the lung adenocarcinoma and adjacent normal lung tissues was assessed by immunoblotting as described in the methods. GAPDH was used as loading control. (b) Semiquantification of the immunoblotting data. Image was analyzed, and density of each band was obtained by the Image J software. Data was an average of three immunoblotting bands obtained in three separate experiments. GAPDH was used as internal control, and data was expressed as percent versus normal (%). (c) Expression of IDO1 mRNA. Quantification of IDO1 mRNA was performed by real-time RT-PCR as described in the method. : adjacent normal lung tissue. I, II, and III: stage I, II, and III adenocarcinoma. .

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