Regulatory Effects of Lycium barbarum Extract and Isolated Scopoletin on Atopic Dermatitis-Like Skin Inflammation

Figure 4

Effect of LBE on proinflammatory cytokine secretion in TNF-α/IFN-γ-stimulated HaCaT cells. HaCaT cells were pretreated with 10, 30, and 60 μg/mL LBE for 1 h before the stimulation. Then, HaCaT cells were stimulated with TNF-α/IFN-γ for 24 h and the supernatant was collected. The levels of secreted proinflammatory cytokines and chemokines were measured by ELISA. compared with TNF-α/IFN-γ-stimulated group.